Stimulated Raman scattering microscopy: From label-free to molecular probes
Thesis Defense in Chemistry
Presented by Yupeng Miao, Min Group
The newly emerging Stimulated Raman Scattering (SRS) Microscopy has been proved to be a powerful tool in biomedical research. This advanced imaging platform offers high spatiotemporal resolution and chemical specificity, which greatly empowers the label-free biomedical imaging and small molecule metabolite tracing. Throughout our new research, we focus on the exploration of more applications of SRS microscopy beyond the aforementioned. Particularly, this new expedition involves more chemistry and answered two major questions: what SRS can do for chemistry and what chemistry can do for SRS. Herein, I will discuss three representative projects. The first one is the application of SRS microscopy in investigating solid-state polymer electrolyte for lithium metal battery (LMB) development, which is an example of applying SRS for materials science. The second one involves SRS microscopy for in-vivo drug tracing in mammalian cells. And the last project heavily focuses on the development of synthetic molecular probes for super-multiplexed optical imaging. Together, these results suggest SRS as a microscopy technique can advance our understanding of chemistry and in return chemistry provides sharpened molecular tools for greater imaging utilities.